Fig. 3

Differential alterations in plasma cytokine content with age and sex. a Multiplex pro-inflammatory cytokine/chemokine analysis of plasma samples from young and aged male and female mice. b Plots show IFN-γ:IL-4 plasma concentration ratios of young and aged males and females. c Plots show TNF-α:IL-10 plasma concentration ratios of young and aged males and females. Young males (n = 8, 2–3 months); young females (n = 5, 2–3 months); aged males (n = 5, 18–19 months); aged females (n = 5, 18–19 months). Data were analyzed by 2-way ANOVA followed by Fisher’s LSD post hoc analysis. Data represent mean ± SEM. Comparisons within sex: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Comparisons within age: #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001