Fig. 4

Age- and sex-dependent changes in cytokine/chemokine concentrations following whole blood stimulation. Whole blood samples were collected from young and aged male and female mice. Samples were either unstimulated or stimulated with 10 ng/mL LPS for 24 hrs. a Plots represent multiplex pro-inflammatory cytokine analysis of young and aged males following whole blood stimulation. b Plots represent multiplex pro-inflammatory cytokine analysis of young and aged females following whole blood stimulation. Young males (n = 3–8, 2–3 months); young females (n = 5, 2–3 months); aged males (n = 5, 18–19 months); aged females (n = 5, 18–19 months). Data were analyzed by 2-way ANOVA with Fisher’s LSD post hoc test. Data represent mean ± SEM. Age: #p < 0.05, ##p < 0.001, ###p < 0.001; Stimulation: *p < 0.05, **p < 0.01, ***p < 0.001