Fig. 3

Islet T cells accumulate during ageing in non-diabetic mice. a-c Islets were isolated from 3- and 24-month old mice. Data represent 3 samples per age group, with islets from 5 to 7 mice pooled per sample. a Islet cells gated on FSC, SSC, viability (7AAD-) and CD45+, representative of 3 independent samples. b-c Quantification of immune cell subsets as a percent of immune (CD45+) cells (b) and total live cells (c) in islets from 3- and 24-month old mice, data represent mean ± SD, and were analyzed by unpaired T-test. d Quantification of immune cell subsets as a percent of total live cells in spleens from 3- and 24-month old mice, n = 3, gated on FSC, SSC, viability (7AAD-) and CD45+. e Transcript abundance of sorted CD3+ islet cells, in 3-month samples (black) and 24-month samples (blue), data represent mean ± SD. f Cell profile analysis was performed on transcriptome profiles obtained from sorted CD3+ islet cells from 3- and 24-month old mice via NSolver4.0, revealing a statistically significant T cell profile relative to other cell types, n = 3 samples, 5–7 mice pooled per sample. Numbers in FACS plots represent the percent of cells in each selection as a function of the parent population. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001