Fig. 6

Weight gain via high fat feeding does not elicit T cell accumulation in islets. C57BL/6 J mice were fed a high fat diet (HFD, purple squares) or low fat diet (LFD, grey circles) for 9–12 weeks from 6 weeks of age. Non-fasted body weight (a) and blood glucose (b) after 9 weeks of diet. Glucose tolerance tests (c-d, n = 5–7), shown as glucose traces (c) and incremental AUC (d), and insulin tolerance tests (e-g, n = 3–5) at 10 and 11 weeks of diet, shown as raw blood glucose (e), delta blood glucose (f), and net AUC relative to baseline (g). h Fasted plasma insulin at 11–12 weeks of diet. Data represent mean ± SEM, and were analyzed by Student’s T-test with Welch’s correction (a,d,g), Mann-Whitney test (b,h), or two-way repeated measures ANOVA with Sidak’s multiple comparisons test (c,e,f). i-j Islets were isolated from LFD- and HFD-fed mice after 12 weeks of diet (n = 3 samples, 5 mice pooled per sample), dispersed, and gated on FSC, SSC, viability (7AAD-) and CD45+, and subsequently CD19 and CD3. i Representative data for LFD- and HFD-mouse islets. j Quantification of islet immune cell populations as a function of viable islet cells, data represent mean ± SD and were analyzed by Mann-Whitney test. Numbers in FACS plots represent the percent of cells in each selection as a function of the parent population