Fig. 4
From: Hyper‐metabolic B cells in the spleens of old mice make antibodies with autoimmune specificities
Total B cells and ABCs from old mice are characterized by a higher metabolic profile as compared to those from young mice. B cells, isolated by magnetic sorting from the spleens of young and old mice, were left unstimulated. Results are representative of 4 pairs of young and old mice. a. Schematic of OCR measured by a Mitostress test conducted in a Seahorse XFp extracellular flux analyzer (left). OCR results in unstimulated B cells from young and old mice (center). ECAR results in unstimulated B cells from young and old mice (right). b. Measures of basal respiration, maximal respiration, spare respiratory capacity, ATP production, proton leak, and non-mitochondrial respiration. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p<0.05, **p<0.01, ***p<0.001. c. OCR (top) and ECAR (bottom) results from in unstimulated FO and ABCs sorted from the spleens of young and old mice