Fig. 4
Multifunctional (MF) and single-expressing (S) S. Typhi-specific CD4+ and CD8+ TEM responses following Ty21a immunization. Net S. Typhi-specific CD4+ and CD8+ TEM responses were calculated using the FCOM function of Winlist and stratified into S and MF responses. Comparison of TI LPMC S. Typhi-specific (a) CD4+ TEM S, (b) CD4+ TEM MF, (c) CD8+ TEM S and (d) CD8+ TEM MF responses between Ty21a-vaccinated (red symbols) and unvaccinated (black symbols) volunteers were determined with significant differences shown (*P < 0.05). Trends to exhibit significance values are indicated by their p-values. Horizontal black bars represent median values