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Fig. 1 | Immunity & Ageing

Fig. 1

From: Local immune responses to tuberculin skin challenge in Mycobacterium bovis BCG-vaccinated baboons: a pilot study of younger and older animals

Fig. 1

An in vivo model of tuberculin recall response in Mycobacterium bovis BCG-vaccinated baboons. a Study timeline: 2 adult and 2 aged baboons were BCG-vaccinated and challenged with tuberculin (TST) or saline (NaCl) for determination of the short term (ST) recall response. Skin biopsies were performed 3 days and 7 days post-challenge. Long term (LT) responses were tested by challenge and biopsies performed at week 25, week 25 + 3 days, and week 25 + 7 days, as indicated. Peripheral blood was collected at the times indicated for PBMC isolation. b Schematic of TST challenges and biopsy sites on the chest of vaccinated baboons. Saline injection sites served as the control for antigen-specific responses. c Representative images of saline (left) and TST (right) injection sites, indicated by blue circle, on the chest of vaccinated baboons, showing positive responses to TST. d Levels of superoxide detected by EPR in adult and aged baboon skin tissue. Shown is the fold change of superoxide in TST biopsies (TST) vs saline (NaCl) biopsies from ST 3-day (left) and 7-day (right) biopsies. e Reduced glutathione (GSH) in ST 3-day (left) and 7-day (right) biopsies, determined by ELISA. f Protein carbonyl levels in ST 3-day (left) and 7-day (right) biopsies, determined by ELISA. g Superoxide in baboon skin tissue in response to TST (fold change vs saline) from LT 3-day (left) and 7-day (right) biopsies. h Protein carbonyl levels in LT 3-day (left) and 7-day (right) biopsies. i GSH in LT 3-day (left) and 7-day (right) biopsies. One-way ANOVA post-Tukey analyses Adult TST vs Aged TST, *p < 0.05

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