Fig. 4

Age related changes in T cell subsets in spleen and bone marrow of SPF mice. a and e Identification of differentially distributed cellular phenotypes by t-SNE in concatenated spleen and bone marrow samples respectively (Young n = 6 and old n = 13). b and f The heatmaps show median marker intensities within each population in spleen and bone marrow respectively. c and g Selected populations of CD4+ T cells. d and h Selected populations of CD8+ T cells. Populations of T cells were gated according to established lineage markers (Supplemental Fig. 1 and 3 for gating strategy). Line indicates mean, ± SD, *P < 0.05, **P < 0.01 ***P < 0.001 Mann-Whitney test. NS indicates non-significant changes