Fig. 6

Young, old SPF and pet shop mice differ in their overall CD45+ splenic leukocyte composition. a Identification of differentially distributed cellular phenotypes by t-SNE in concatenated pet shop spleen samples (n = 4). b The heatmap shows z-scores of population frequencies of major immune population identified in spleen between all three mouse groups. Heat map coloring has been scaled per row, higher than mean (red) and lower than mean (blue). Each cluster of correlating populations (dashed boxes), features immune signatures that distinguish the three experimental groups. c Selected naïve and differentiated immune populations in the spleen characteristic among all three mouse groups. Line indicates mean, ± SD, *P < 0.05, **P < 0.01 ***P < 0.001 Mann-Whitney test