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Fig. 4 | Immunity & Ageing

Fig. 4

From: Hyperactivation of monocytes and macrophages in MCI patients contributes to the progression of Alzheimer's disease

Fig. 4

Signaling in monocytes in the resting state obtained from various patient groups. a p42/44 ERK and p-p42/44 expression was determined in total protein extracts by Western blot analysis, using anti-p42/44 and anti-p-p42/44 antibodies. Results are shown as means of p-p42-44/p42-44 ratio ± SEM and normalized to the total expression at healthy individuals. Dunnett’s multiple comparison test; *p < 0.05. NS: non-significant, n=5. b NF-kB and p-NF-kB expression was determined in total protein extracts by Western blot analysis, using anti-NF-kB and anti-p-NF-κB antibodies. Results are shown as means of p-NF-κB/NF-κB ratio ± SEM and normalized to the total expression of healthy individuals. Dunnett’s multiple comparison test; *p < 0.05. NS: non-significant; n=5. c IRF3 and p-IRF3 expression was determined in total protein extracts by Western blot analysis, using anti-IRF3 and anti-p-IRF3 antibodies. Results are shown as means of p-IRF3/IRF3 ratio ± SEM and normalized to the total expression of healthy individuals. Dunnett’s multiple comparison test; NS: nonsignificant; n=5. d STING and p-STING expression was determined in total protein extracts by Western blot analysis, using anti-STING and anti-p-STING antibodies. Results are shown as means of p-STING/STING ratio ± SEM. Dunnett’s multiple comparison test; NS: nonsignificant; n=5. e MyD88 expression was determined in total protein extracts by Western blot analysis, using an anti-MyD88 antibodies. Actin (total) was used to normalize the data. Dunnett’s multiple comparison test; NS: nonsignificant; n=5. f NOD2 expression was determined in total protein extracts by Western blot analysis, using anti-NOD2 antibodies. Actin (total) was used to normalize the data. Dunnett’s multiple comparison test; NS: nonsignificant; n=5. e MyD88 expression was determined in total protein extracts by Western blot analysis, using an anti-MyD88 antibodies. Actin (total) was used to normalize the data. Dunnett’s multiple comparison test; NS: nonsignificant; n=5. f NOD2 expression was determined in total protein extracts by Western blot analysis, using anti-NOD2 antibodies. Actin (total) was used to normalize the data. Dunnett’s multiple comparison test; NS: nonsignificant; n=5.

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