Fig. 1

Alteration in Gene Expression Profile of Articular Cartilage in Zmpste24-deficient (Zmpste24^−/−) Progeroid Mice. Articular cartilage from 5–6 month-old adult wild type (n = 6) and age-matched Zmpste24^−/− littermate knee joints (n = 5) were analyzed for the mRNA expression level of (a-c genes encoding pro-inflammatory cytokines and senescence-associated secretory phenotype (SASP) factors (Il1a, Il6, and Tnf), extracellular matrix (ECM) proteinases (Mmp3, Mmp13, and Adamts5), ECM components (Col1a1, Col2a1, Acan, Vcan, and Bgn) using qRT-PCR. d-e Genes associated with telomere protection (Pot1b), autophagy suppression (Mtor), antioxidant response (Gpx4), and oxidative stress-response (Sod1). Expression values are relative to housekeeping genes, Gapdh and Hmgb1. Data are mean ± SEM. **p < 0.01, ***p < 0.001, ^#p < 0.0001, ^ǂp < 0.000001, ns: not significant using two-tailed, unpaired Student’s t-test or Welch’s unequal variance t-test