Fig. 4

Moderate ethanol exposure in aged mice leads to increased neutrophils and monocytes in the liver.Flow cytometric analysis of hepatic non-parenchymal cells from aged and young mice given vehicle or ethanol. A Representative density plots showing gating strategy to define hepatic neutrophil, Kupffer Cell (KC), and infiltrating macrophage (IM) and monocyte populations. B Representative density plots showing the percent of hepatic CD45⁺ cells that are neutrophils. C-F. Bar charts showing the percent of CD45⁺ cells that are neutrophils, Kupffer cells, infiltrating macrophages and monocytes in the indicated treatment groups. n = 4–8 mice per group. Data shown are mean ± SEM. *p < 0.05 from all other groups by two-way ANOVA with Tukey’s multiple comparisons test