Fig. 2

BDNF-AS acted as a sponge for miR-125a/b-5p. a The putative binding site between BDNF-AS and miR-125a/b-5p was predicted by Starbase 3.0. b HEK-293 T cells were transfected with miR-125a/b-5p mimics or inhibitors. The expression of miR-125a-5p and miR-125b-5p was detected by qRT-PCR. c The relative enrichment of BDNF-AS in HEK-293 T cells was determined by RIP assay. d The relative luciferase activity of BDNF-AS-WT or BDNF-AS-MUT in HEK-293 T cells was detected by dual luciferase reporter system. e HEK-293 T cells were transfected with the miR-125a/b-5p mimics or inhibitors. The expression of BDNF-AS was detected by qRT-PCR. f HEK-293 T cells were transfected with BDNF-AS shRNA or overexpression vector, and the expression of miR-125a/b-5p was detected by qRT-PCR. **p < 0.01, ***p < 0.001. ns: not significant