Fig. 3

Knockdown of BDNF-AS significantly reduced proliferation of MM cells. MM.1S and U266 cells were transfected with the lentiviral vector carrying sh-BDNF-AS and sh-NC. a The expression of BDNF-AS was detected by qRT-PCR. b Cell viability was evaluated by CCK-8 assay. c Cell migration ability was evaluated by wound healing assay. Scale bar = 100 μm. d Cell proliferation was evaluated by EdU staining assay. Scale bar = 30 μm. e The expression of apoptosis-related proteins was evaluated by Western blot. Relative cleaved caspase-3 means cleaved caspase-3/total caspase-3, and relative cleaved PARP means cleaved PARP/total PARP. **p < 0.01, ***p < 0.001