Fig. 6

Bcl-2 was a target of miR-125a-5p and miR-125b-5p. a The putative binding site between miR-125a/b-5p and Bcl-2 was predicted by Starbase 3.0. b The relative luciferase activity of Bcl-2-WT or Bcl-2-MUT in HEK-293 T cells was detected by dual luciferase reporter system. c The enrichment of BCL-2 in HEK-293 T cells was detected by qRT-PCR. d The expression of Bcl-2 in MM.1S and U266 cells was detected by Western blot. e MM.1S and U266 cells were transfected with lentiviral vectors carrying sh-Bcl-2 and sh-NC, and the transfection efficiency was detected by qRT-PCR. f MM.1S and U266 cells were transfected miR-125a-5p inhibitor, miR-125b-5p inhibitor, or co-transfected with miR-125a-5p inhibitor and sh-Bcl-2, or co-transfected with miR-125b-5p inhibitor and sh-Bcl-2. Cell viability was evaluated by CCK-8 assay. *p < 0.05, **p < 0.01, ***p < 0.001 vs miR-NC group/miR-125a/b-5p inhibitor group; #p < 0.05 vs sh-NC group. ns: not significant