Fig. 8

Knockdown of BDNF-AS suppressed tumor growth in vivo. a Tumor volume was evaluated every week for 4 weeks. b Tumor weight was evaluated after 4 weeks. c Representative images of xenograft tumors. d The expression of Ki-67 in tumor tissues was evaluated by IHC staining. Scale bar = 30 μm. e and f The expression of BDNF-AS (e) and miR-125a/b-5p (f) in tumor tissues was detected by qRT-PCR. (g) The expression of Bcl-2 in tumor tissues was detected by Western blot. (H) The levels of cleaved caspase 3 (c-caspase 3), total caspase 3 (t-caspase 3), cleaved PARP (c-PARP) and total PARP (t-PARP) in tumor tissues was evaluated by Western blot. Four mice in each group. Relative cleaved caspase-3 means cleaved caspase-3/total caspase-3, and relative cleaved PARP means cleaved PARP/total PARP. *p < 0.05, **p < 0.01, ***p < 0.001