Fig. 4
From: The immunosenescence-related factor DOCK11 is involved in secondary immune responses of B cells
Impact of the DOCK11 deficiency in cytokine production of B cells. A, The levels of IL-6, TNFα, TGFβ, and IL-10 in cell culture supernatant were compared between WT and Dock11-deficient B cells 72 h after stimulation of 20 μg/ml anti-IgM F (ab’)2 antibody or 10 μg/ml LPS as well as no stimulation. B, Viability of each column was calculated by dividing the number of propidium iodide (PI)-negative cells by that of total cells after staining the cells with 2 μg/ml of PI and counted 1 × 104 cells with flow cytometry. Data are mean ± SEM of triplicate wells. **P < 0.01, *P < 0.05 (two-tailed unpaired Student’s t-test)