Fig. 1
From: Impaired JAK-STAT pathway signaling in leukocytes of the frail elderly
Study and experimental design. CMV-seronegative participants were selected from the Doetinchem cohort study. PBMCs were thawed and stimulated with the indicated cytokines or were left unstimulated (baseline). Intranuclear immunofluorescence staining was performed to quantify phosphorylation of STAT1, 3, and 5 proteins, in combination with surface staining for lineage-specific markers to distinguish between B cells, monocytes, and T cells (CD8+ and CD4+)