Fig. 4

Effect of ACA, Rapa and 17aE2 treatments on liver MNK signaling in liver. A Representative western blots for phospho-MNK at Threonine 197 (pMNK) and total levels of MNK1 and MNK2 protein in liver samples from young untreated (Y), old untreated (O), old treated with ACA or Rapa (O, early and late) and 17aE2 (early and late) treatments separated by sex (males = M, females = F). B Bar graphs represent the mean ± SEM of pMNK and MNK1 and MNK2 protein levels in liver of 8 young and 8 old male mice plus, at least, 4 mice for each of the ACA and Rapa and 17aE2 male treated groups. All values have been normalized to young control females as described in Methods section. C Bar graphs represent the mean ± SEM of pMNK and MNK1 and MNK2 protein levels in liver of 8 young and 8 old female mice plus, at least, 4 mice for each of the ACA and Rapa and 17aE2 female treated groups. The (*) indicates statistical significance (p < 0.05) in a t-test between the indicated pair of groups