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Fig. 7 | Immunity & Ageing

Fig. 7

From: Rapamycin, Acarbose and 17α-estradiol share common mechanisms regulating the MAPK pathways involved in intracellular signaling and inflammation

Fig. 7

Effect of ACA, Rapa and 17aE2 treatments on MK2 signaling in liver. A Representative western blots for phospho-MK2 at Threonine 334 (pMK2), total levels of MK2 (Short) and MK2 (Long) protein isoforms in liver samples from young untreated (Y), old untreated (O), old treated with ACA or Rapa (O, early and late treatments). B Representative western blots of 17aE2 (early and late) effects in pMK2 and MK2 isoforms separated by sex (males = M, females = F). C Bar graphs represent the mean ± SEM of pMK2 ratios and MK2 (Long and Short) protein levels in liver (top panels) and kidney samples (bottom panels) obtained from 16 young and 16 old mice plus, at least, 8 mice for each of the ACA and Rapa treatments groups. 17aE2 was from 8 young males and 8 young females, 8 old males and 8 old female mice, while treatments represent a minimum of 4 mice for each treatment and sex group. All values have been normalized to young control females as described in Methods section. The (*) indicates statistical significance (p < 0.05) in a t-test between the indicated pair of groups

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Immunity & Ageing

ISSN: 1742-4933

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