Fig. 8

Effect of ACA, Rapa and 17aE2 treatments on the levels of Acute Phase Proteins in liver. A Representative western blots for SAP, HMOX2 and Cas6 in liver samples from young untreated (Y), old untreated (O), and old treated with ACA, Rapa and 17aE2 (O, early and late treatments). B Bar graphs represent the mean ± SEM of SAP, HMOX2 and Cas6 protein in liver samples obtained from 16 young and 16 old mice plus, at least, 8 mice for each of the ACA, Rapa and 17aE2 treatments groups. Values been normalized to young control females for each protein, as described in Methods section. The (*) indicates statistical significance (p < 0.05) in a t-test between the indicated pair of groups. C The scatter plot shows a comparison of CT values and mean ± SEM (horizontal bar) of each mRNA between the groups from 16 young and 16 old mice plus a minimum of 8 mice treated with ACA, Rapa and 17aE2 (earl or late) per group. Each symbol represents a different mouse. The (*) indicates statistical significance (p < 0.05) in a t-test between the indicated pair of groups