Fig. 2

mTerc−/− CD4+ T cells show functional differences after in vitro polarization. A Schematic representation of the experimental setup of in vitro polarization. CD4+ T cells were isolated and stimulated in vitro for five days with anti-CD3 and anti-CD28 only (Th0) or with anti-CD3, anti-CD28, IL-12 and anti-IL-4 (Th1). On the day of analysis, the cells were treated with eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (Invitrogen) for 3.5 h before analysis. B Quantification of IFNγ in the medium by ELISA. C and D Flow cytometric analysis of IFNγ-producing CD4+ T cells. Graphs show the mean ± SD, * adjusted p ≤ 0.05, ** adjusted p ≤ 0.01, *** adjusted p ≤ 0.001