Fig. 4

mTerc−/− CD4+ T cells are more susceptible to IL-6-mediated inhibition of Th1 differentiation. CD4+ T cells were isolated from mTerc−/− mice as before and stimulated in vitro for five days with anti-CD3 and anti-CD28 only (Th0) or with anti-CD3, anti-CD28, IL-12 and anti-IL-4 (Th1). In addition, IL-6 was added to the Th1-inducing mixture in the indicated concentrations. A Flow cytometric analysis of IFNγ-producing cells after in vitro polarization under IL-6 treatment. The figure depicts pooled data from two independent experiments. B Quantification of IFNγ in the medium after in vitro polarization of mTerc−/− CD4+ T cells. The figure shows pooled data from two independent experiments. C Memory populations after in vitro polarization as determined by flow cytometry (effector/ effector memory: CD44+ CD62L-; central memory: CD44+ CD62L+). Data from one measurement is shown. All graphs show the mean ± SD, * adjusted p ≤ 0.05, ** adjusted p ≤ 0.01, *** adjusted p ≤ 0.001, **** adjusted p ≤ 0.0001