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Fig. 1 | Immunity & Ageing

Fig. 1

From: NKT-like (CD3 + CD56+) cells differ from T cells in expression level of cellular protective proteins and sensitivity to stimulation in the process of ageing

Fig. 1

Gating strategy performed for flow cytometric analysis of T lymphocytes (CD3+ cells) and NKT-like cells (CD3 + CD56+ cells). a. Lymphocyte gating in the population of PBMCs – lymphocytes were defined as FSClow/SSClow cells (G1). b. T and NKT-like cell gating – T lymphocytes were defined as the CD3+ positive population (G2), and NKT-like cells were defined as CD3 + CD56+ positive cells (G3). c. T cells expressing SIRT1 were identified in the upper left quadrant (Q1). d. NKT-like cells expressing SIRT1 were identified in the upper right quadrant (Q2). e. Isotype control for SIRT1-positive T cells. f. Isotype control for SIRT1-positive NKT-like cells. The analysis of HSP70 and SOD2 expression was performed in the same way. All age groups were analyzed with the same gating strategy

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