Fig. 1

FoodborneLm infection elicits a robust and diverse CD44^hi CD27^neg Vγ1.1^neg Vγ2^neg γδ T cell response with aging. B6 mice were foodborne infected with OVA-expressing Lm. MLN were harvested and CD44^hi CD27^neg Vγ1.1^neg Vγ2^neg γδ T cells were analyzed by flow cytometry 9 days later. A Schematic of the experimental protocol. B Gating strategy used for the identification and calculation of the frequency of CD44^hi CD27^neg Vγ1.1^neg Vγ2^neg γδ T cells among total γδ T cells is depicted. C Representative flow plots for the indicated groups are shown. Compounding frequency among total γδ T cells (D) and absolute numbers (E) of CD44^hi CD27^neg Vγ1.1^neg Vγ2^neg γδ T cells are shown as mean ± SEM. F Linear regression between CD44^hi CD27^neg Vγ1.1^neg Vγ2^neg γδ T cell numbers and age is shown. G Linear regression between CD44^hi CD27^neg Vγ1.1^neg Vγ2^neg γδ T cell numbers and weight is shown. H Body weight for each age group is shown as mean ± SEM. I Linear regressions between CD44^hi CD27^neg Vγ1.1^neg Vγ2^neg γδ T cell numbers and weight within indicated age groups are shown. J CD44^hi CD27^neg Vγ1.1^neg Vγ2^neg γδ T cell functional was analyzed at 9 days post-infection. Data show the mean ± SEM of cytokine-producing cells. Each symbol represents an individual mouse. All data sets are compiled from at least 3 independent experiments with a minimum of 3 mice/group, except 25-26-month-old B6 (1 experiment, 7 mice) and 7-10-month-old in (H) (1 experiment, 4 mice)