Fig. 1
Young and aged mice differ in outcomes to Lm infection numbers of homeostatic CD8+ bTRM. 2 mo C57BL/6 J (●) and 24 mo C57BL/6JN (■) mice infected i.p. with Lm EGD 2.0 × 105 CFU (n = 9) and 0.3–0.6 × 105 CFU (n = 12), respectively (A, B, and D). All mice received antibiotics 2-16d p.i. Weight was measured daily and is shown as the mean % starting weight for surviving mice (A). Difference in weight loss between cohorts was calculated by 2-way ANOVA. Data in (B) show Kaplan-Meier survival plots of mice aged 2 mo (solid line) and 24 mo (broken line) after infection, significance calculated via log-rank test. Panels C and D show numbers of CD8+ bTRM cells/brain in uninfected 3 mo C57BL6/J (Ο), 9 mo C57BL/6 N (△), 22–23 mo C57BL/6JN (□) and 27 mo C57BL/6JN (▽) male mice (C), and in 24mo old C57BL/6JN male mice 29d p.i. with 0.7 × 105 CFU Lm (■) and uninfected (□) age-matched mice treated with antibiotics (D). Symbols represent individual animals. Statistical analysis by 1-way Brown-Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons post-test (C) or by simple t-test (D)