Fig. 2
Aging alters homeostatic and post-infection gene expression in brain CD11b+ cells. CD11b+ brain cells were magnetically sorted from Young (2 mo) C57BL/6 J and Aged (24 mo) C57BL/6JN mice. Uninfected mice (A) were not manipulated. Infected mice (B) were injected i.p. with Lm EGD 2.0 × 105 CFU (young) or 0.3–0.6 × 105 CFU (aged), then received antibiotics 2d-16d p.i. and were euthanized 29d p.i. Gene expression was measured by nCounts. FDR of q < 0.05 was determined by multiple t-test followed by two-stage step up (Benjamini, Krieger, and Yekutieli) correction for multiple comparisons. Heat maps were based on the log2 fold change (aged/young) nCounts from significantly changed genes