Fig. 1

Age-dependent susceptibility to grass carp reovirus (GCRV) and global methylation profile of the two fish groups. A The cumulative mortality of the two grass carp groups after GCRV infection. B Global DNA methylation level of two grass carp groups. Spleen samples from the two groups were obtained, and the global DNA methylation level (5-mc) was analyzed. Data are shown as the mean ± standard deviation of three replicates. C The percentage of methylcytosines in CG, CHG, and CHH contexts. D Logo plots of the sequences proximal to sites of mCG, mCHG, and mCHH contexts. E Distribution of DNA methylation levels in the two groups. The y axis indicates the fraction of all mCs that display each methylation level (x axis), where the methylation level is the mC/C ratio at each cytosine