Fig. 2

‘Greenyellow’ module of co-expressed genes (n = 298) demonstrating earlier induction in adult mice than aged mice. Heatmap of scaled gene expression and eigengene barplot (A) show adults have generally earlier induction for most genes and samples. Each row of the heatmap represents an individual gene, while each column represents an individual sample. The barplot displays the single eigengene value for that sample, which is essentially a 1.^st principal component ‘gene’ of the module. The interaction plot (B) shows the behavior of the top 30 genes by their module membership. Each line is a separate gene, and the same genes are shown for both age classes. The samples from adult mice (black lines) are up-regulated one two-day interval earlier than in aged mice (greenyellow lines). A full table of module membership is shown in Supplementary Table S1. The order of timepoints within each age class is: 0, 1, 3, 5, 7, and 9 days post-infection (n = 34 total)