Fig. 6

Transcriptional regulation of chemotaxis-related genes in human microglia after treating with CSF from the elderly. a Sorted primary human microglia were cultured with CSF from the elderly (n = 4) or PBS (n = 2) for 24 h and then collected for RNA sequencing. b mRNA expression levels of microglia (AIF1,TMEM119), neurons (MAP 2, NCAM1), Astrocytes (GFAP, ALDH1L1), Oligodendrocytes (APC, PLP1), Endothelial cells (CLDN5, ITM2A), T cells (CD3E) and B cells (CD79A) marker genes. c Volcano plot depicting the differentially expressed genes in CSF treated microglia compared to PBS treated microglia. Differentially expressed genes were colored (red for upregulated DEGs and green for downregulated DEGs). d Functional enrichment analysis was performed using Metascape on the upregulated DEGs in (c). The significantly overrepresented (P < 0.01) terms were grouped into color-coded clusters based on their membership similarities and rendered as a network plot. Each node represents an enriched term, and one representative term is shown for each cluster. Terms with a similarity > 0.3 are connected by edges. e Expression profiles of DEGs in CSF treated microglia related to leukocyte recruitment pathways