Fig. 4

Cluster analysis of lymphocyte parameters. A The t-SNE analysis using 35 parameters (17 lymphocyte indicators: CD3⁺ T cell percentage, CD3⁺CD4⁺ T cell percentage, CD3⁺CD8⁺ T cell percentage, CD3⁻CD19⁺ B cell percentage, CD3⁻CD16⁺CD56⁺ NK cell percentage, CD3⁺ T cell number, CD3⁺CD4⁺ T cell number, CD3⁺CD8⁺ T cell number, CD3⁻CD19⁺ B cells number, CD3⁻CD16⁺CD56⁺ NK cell number, CD28⁺CD4⁺ T cell percentage, CD28⁺CD8⁺ T cell percentage, CD45RA⁺CD4⁺ T cell percentage, HLA-DR⁺CD8⁺ T cell percentage, IFN-γ⁺CD4⁺ T cell percentage, IFN-γ⁺CD8⁺ T cell percentage, and CD4/CD8; 18 routine parameters: age, BMI, WBC, NEUT, LYMPH, MONO, EOSIN, BASO,RBC, HB, PLT, ALB, GLB, TC, TG, HDL, LDL, and GLU) to clarify the regional difference of healthy adults among centers. B Hierarchical cluster analysis of lymphocyte and routine parameters together. The three red boxes represent 3 clusters. Dendrograms are shown as trees, representing the distance between variables. t-SNE, t-distributed stochastic neighbor embedding; BMI, body mass index; WBC, white blood cell; NEUT, neutrophil; LYMPH, lymphocyte; MONO, monocyte; EOSIN, eosinophil; BASO, basophil; RBC, red blood cell; HB, hemoglobin; PLT, platelet; ALB, albumin; GLB, globulin; TC, total cholesterol; TG, triglyceride; HDL, high density lipoprotein; LDL, low density lipoprotein; GLU, glucose