Fig. 1

Frequency and differentiation status of IAV- and CMV-specific CD8⁺ T cells in blood of CMV infected healthy adults. A Gating of CMV-NLV (pp65_495–503) or IAV-GIL(M1_58-66) -specific CD8⁺ T cell subsets. CMV- and IAV-specific cells from the same donor were further classified into T_N (CD45RA⁺CD62L⁺CD95^-), T_SCM (CD45RA⁺CD62L⁺CD95⁺), T_CM (CD45RA^-CD62L⁺), T_EM (CD45RA^-CD62L^-), and T_EMRA (CD45RA⁺CD62L^-). B Frequencies of CMV- or IAV-specific CD8⁺ T cells in CMV IgG positive (CMV infected) and negative young and middle-aged (<65-year-old) healthy adults (N=18 each, age- and sex-matched). C Frequencies of CMV- and IAV-specific CD8⁺ T cell subsets in CMV IgG positive young and middle-aged healthy adults (N=25). D Percentages of CD127⁺, CD27⁺CD28⁺, and Granzyme B (GZB)⁺ perforin⁺ cells in CMV- specific and IAV-specific CD8⁺ T cell subsets from CMV IgG positive young and middle-aged healthy adults (N=20). Data represent the mean values ± standard error of the mean (SEM). * Indicates P < 0.05, ** indicates P < 0.01