Skip to main content
Fig. 6 | Immunity & Ageing

Fig. 6

From: Aging beyond menopause selectively decreases CD8+ T cell numbers but enhances cytotoxic activity in the human endometrium

Fig. 6

Aging and menopause differentially regulate the production of TNFα, IL-6 and IFNγ by EM CD103+ and CD103-CD8+ T cells after activation. Purified EM CD103+ and CD103-CD8+ T cells were stimulated with PMA (100 ng/ml) and ionomycin (2 μM) for 24 h. The culture supernatants were collected and assayed for TNFα, IL-6 and IFNγ by Luminex assay. A Production of TNFα, IL-6 and IFNγ by EM CD103+ (grey circle) and CD103- (black square) CD8+ T cells after 24 h stimulation (n = 20). C represents untreated control. S represents stimulation with PMA/ionomycin. B Comparison of pre- (black circle; n = 13) vs. post-menopausal (white circle; n = 7) women production of TNFα, IL-6 and IFNγ by EM CD103+ (left) and CD103- (right) CD8+ T cells after 24 h stimulation. C, D Correlation between age and the production of TNFα, IL-6 and IFNγ by EM CD103 + CD8+ T cells (C) and CD103-CD8+ T cells (D) after 24 h stimulation. Graphs of (B-D) represent the fold change in TNFα, IL-6 and IFNγ in culture supernatant following stimulation compared with untreated controls (n = 20). Each dot represents a single patient. Mean ± SEM are shown. *P < 0.05, **P < 0.01, ****P < 0.0001; Friedman test followed by Dunns post-test (A), Kruskal-Wallis test followed by Dunns post-test (B), Spearman test (C, D)

Back to article page