Fig. 1
From: Phenotypic and functional alterations of monocyte subsets with aging
The cytokine profiles of total monocytes in healthy adults in different age groups. A. Comparison of the percentage of total monocytes among peripheral blood mononuclear cells (PBMCs) in young, middle-aged, and older adults via flow cytometry (young: 21–40 years, n = 42; middle-aged: 41–60 years, n = 34; older: > 60 years, n = 34). Data are shown as a box plot with medians (lines inside boxes), 25th, and 75th quartiles (limits of boxes). Whiskers indicate the range, and each dot represents one sample. P-values were obtained using a Kruskal–Wallis test, followed by post hoc analysis. B. Representative flow cytometry data of intracellular staining for TNF-α, IL-6, IL-10, GM-CSF, and IL-1β in total monocytes stimulated with LPS (100 ng/mL) for 3 h in vitro from young, middle-aged, and older adults (young: 21–40 years, n = 42; middle-aged: 41–60 years, n = 34; older: > 60 years, n = 34). C. Intracellular staining for the percentage of TNF-α+ and IL-6+ monocytes stimulated with LPS (100 ng/mL) for 3 h in vitro from young, middle-aged, and older adults (young: 21–40 years, n = 42; middle-aged: 41–60 years, n = 34; older: > 60 years, n = 34) upon in vitro LPS stimulation. Data are shown as box-plots with medians (lines inside boxes), 25th, and 75th quartiles (limits of boxes). Whiskers indicate the range, and each dot represents one sample. P-values were obtained by a Kruskal–Wallis rank test, followed by post hoc analysis