Fig. 1

Single-cell clustering analysis of PBMCs from mice to evaluate changes in immune cell proportions during GA treatment. A Schematic of the experimental design for single-cell sequences. PBMCs collected from young (n = 5), aged (n = 5), and aged + GA (n = 5) mice were processed by scRNA-seq using a 10 × genomics platform. B Clustering of single cells in aged mice. C Clustering of single cells in aged + GA mice. D Comparison of clustering of single cells in aged and aged + GA mice. E Relative population abundances of cell types in aged and aged + GA mice. F KEGG analysis of differentially expressed genes in Cluster 0 (B cells, Cd74 as marker gene). G KEGG analysis of differentially expressed genes in Cluster 8 (T cells, Ccl5 as marker gene). H KEGG analysis of differentially expressed genes in Cluster 9 (T cells, Cd8b1 as marker gene)