Fig. 4

Glycyrrhizic acid promoted differentiation of Lin⁻CD117⁺ HSCs into CD8⁺ T cells in vitro. A Experimental design of Lin⁻CD117⁺ HSCs co-cultured with OP9-DL1 cells for long-term directed differentiation into T cells. B Representative FACS plots and bar graphs of T-cell DN phase changes (DN1 phase, CD44⁺CD25⁻; DN2 phase, CD44⁺CD25⁺; DN3 phase, CD44⁻CD25⁺; DN4 phase, CD44⁻CD25⁻; and DP phase, CD8⁺CD4⁺) in co-culture differentiation of Lin⁻CD117⁺ HSCs and OP9-DL1 cells. C Representative FACS plots and bar graphs of CD8⁺ T cells and CD4⁺ T cells in co-culture differentiation of Lin⁻CD117⁺ HSCs with OP9-DL1 cells. Data represent mean ± SEM. n = 3, *P < 0. 05, **P < 0.01, ***P < 0.001; ns, not significant vs. Ctrl