Fig. 6

Co-culture differentiation of modified Lin⁻CD117⁺ HSCs with OP9/OP9-DL1 cells. A Experimental design of co-culture differentiation of Lin⁻CD117⁺ HSCs with OP9 cells for 6 d in LV201, LV201 + GA, OEa8 (LV201 + S100A8), and OEa8 + GA (LV201 + S100A8 + GA) groups. B Representative FACS plots of CD11b⁺ cells in co-cultures of Lin⁻CD117⁺ HSCs with OP9 cells in LV201, LV201 + GA, OEa8, and OEa8 + GA groups. C Bar graph showing CD11b⁺ cells in co-cultures of Lin⁻CD117⁺ HSCs with OP9 cells in LV201, LV201 + GA, OEa8, and OEa8 + GA groups. D Experimental design of co-culture differentiation of Lin⁻CD117⁺ HSCs with OP9-DL1 cells for 6 d in LV201, LV201 + GA, OEa8, and OEa8 + GA groups. E Representative FACS plots of CD11b⁺ cells in co-cultures of Lin⁻CD117⁺ HSCs with OP9-DL1 cells in LV201, LV201 + GA, OEa8, and OEa8 + GA groups. F Bar graph showing CD11b⁺ cells in co-cultures of Lin⁻CD117⁺ HSCs with OP9-DL1 cells in LV201, LV201 + GA, OEa8, and OEa8 + GA groups. Data represent mean ± SEM, n = 3, *P < 0.05