Fig. 2

Single cell transcriptional profiling identified five AM populations in aged and young mice. AMs (CD45⁺ F4/80⁺ CD11c⁺) purified from lung MNCs (10 mice/sample) were analyzed through single cell RNA-seq. A Uniform manifold approximation and projection (UMAP) plots showed cell clustering based on gene expression. Five transcriptionally distinct clusters (C1-C5) were identified for the AMs in aged and young mice. B Heatmap showing the top 15 marker genes for each cluster (C1-C5). C1 marker genes are shown in larger font. C mRNA expression levels of functional molecules in each cluster (C1-C5) were compared between aged and young AMs