Fig. 3

Aged AMs were featured with distinguished differentiation states compared with young AMs. Pseudotime trajectory analysis of AMs for the indicated groups. A Pseudotime trajectory analysis of AMs from the aged and young mice group (aged, n = 11,944; young, n = 7568) was conducted using Monocle 2. Components 1 and 2 were shown, and each dot represented a single cell. Dots were colored according to their state (left) and cell cluster (right). B Percentages and cell numbers of AMs in the indicated states were shown in a table. C Percentages of AMs in the indicated states were shown as a bar graph. D Statistical analysis of the percentages of AMs in the indicated states between the aged and young mice. A Chi-square test was used. *p < 0.05, **p < 0.01