Table 2 Antibodies used for flow cytometry immune cell phenotyping in human peripheral blood mononuclear cells (PBMCs)
From: The effect of metformin on influenza vaccine responses in nondiabetic older adults: a pilot trial
Antibody | Fluorochrome | Clone | Manufacturer |
|---|---|---|---|
CD3 | BV 605 | OKT3 | Biolegend |
CD4 | BUV 496 | SK3 | BD Biosciences |
CD8 | BUV 737 | SK1 | BD Biosciences |
CD57 | PE-Dazzle 594 | HNK-1 | Biolegend |
ICOS | BV 421 | C398.4A | Biolegend |
PD-1 | PE | EH12.2H7 | Biolegend |
CXCR5 | BV 510 | RF8B2 | BD Biosciences |
CD197 (CCR7) | APC-Fire 750 | GO43H7 | Biolegend |
CD45RA | PE-Cy7 | HI100 | Biolegend |
CD19 | BUV 661 | H1B19 | BD Biosciences |
CD24 | BV 510 | ML5 | Biolegend |
CD27 | BV 605 | M-T271 | Biolegend |
CD38 | BV 421 | H1T2 | Biolegend |
IgD | PerCP-Cy5.5 | IA6-2 | Biolegend |
Carboxylic acid, succinimidylester (live/dead) | Alexa Fluor 350 | Â | Life Technologies |