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Fig. 1 | Immunity & Ageing

Fig. 1

From: Hemagglutinin virus-like particles incorporated with membrane-bound cytokine adjuvants provide protection against homologous and heterologous influenza virus challenge in aged mice

Fig. 1

Development and characterization of influenza A/PR8 HA-VLP and A/PR8 HA VLP-cytokines modified VLP vaccine. A SDS-PAGE gel colloidal blue staining of PR8 HA-VLP expressed in insect cells after purification, displaying HA and M1 proteins. B GPI-cytokines incorporation schema for HA-VLPs (created with Biorender.com). C Flow cytometry analysis of HA-VLP (control) and HA-VLP-Cytokines vaccine incorporated GPI-GM-CSF and GPI-IL-12 (HA-VLP-Cyt). D Western blot analysis of incorporated GPI-GM-CSF or GPI-IL-12 in HA-VLPs using anti-mouse GM-CSF antibody and anti-mouse IL-12 p40 antibody respectively. PR8 HA-VLP contains HA from A/PR8/34, PR8 HA-VLP-Cyt contains HA and cytokines (GM-CSF and IL-12). Size markers are shown only for the region of interest

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Immunity & Ageing

ISSN: 1742-4933

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