Fig. 5

Aging-dependent alterations in T cells. A. Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP)plot of T cells, subclusters (B), and the cell distribution in 3 months and 21 months old samples (C). D. Alterations in cell proportions of T cell subclusters. E. Expression patterns of marker genes used for cell type annotation. The fraction of cells that expressed the marker genes is indicated by the size of the circle, and the means of the expression levels of marker genes are indicated by the color. F. Cell markers of T0. G. Immunohistochemical staining for CD45 in the epididymal initial segment of young and old mice. H. Funnel plot shows the genes with downregulated and upregulated expression in the principal cells during aging. The top 10 genes with upregulated and downregulated expression are marked. I. Gene Ontology terms of differentially expressed genes with upregulated expression in T0. J. Overlapping genes with downregulated or upregulated expression in the aged T0 subtype and included in the Aging atlas database