Immunity & Ageing

Table 1 Comparison of mononuclear cells from different sources of BM and from peripheral blood (PB)

From: Bone marrow T cells from the femur are similar to iliac crest derived cells in old age and represent a useful tool for studying the aged immune system

	BM			PB
CD4⁺	Femur	Iliac crest	P^#
CD4⁺	(n = 8)	(n = 8)		(n = 16)
% CCR7⁺CD45RA⁺ (T_N)	26.1 ± 2.3	19.8 ± 1.9	0.08	34.8 ± 2.9^*/§
%CCR7⁺CD45RA^- (T_CM)	21.9 ± 5.9	24.4 ± 3.1	0.89	28.8 ± 3.7
% CCR7^-CD45RA^- (T_EM)	34.6 ± 4.4	44.3 ± 3.6	0.11	23.8 ± 3.3^*/§
%CCR7^-CD45RA⁺ (T_EMRA)	12.1 ± 3.3	13.4 ± 3.0	0.80	15.7 ± 2.7
CD69	16.9 ± 3.1	15.9 ± 2.3	0.69	0.7 ± 0.1^*/§
CD8 ⁺	Femur	Iliac crest
CD8 ⁺	(n = 8)	(n = 8)		(n = 16)
% CCR7⁺CD45RA⁺ (T_N)	17.8 ± 3.5	14.1 ± 3.2	0.47	29.3 ± 3.4^*/§
%CCR7⁺CD45RA^- (T_CM)	16.7 ± 3.1	10.9 ± 4.1	0.68	14.5 ± 2.2
% CCR7^-CD45RA^- (T_EM)	29.1 ± 4.2	34.1 ± 6.7	0.64	21.9 ± 2.6^*/§
%CCR7^-CD45RA⁺ (T_EMRA)	33.0 ± 0.5	41.6 ± 5.9	0.59	39.5 ± 3.7
CD69	39.0 ± 6.1	46.3 ± 4.1	0.59	1.9 ± 0.4^*/§

BM was obtained either from the femur shaft or from iliac crest, as described in Materials and Methods. All samples were from elderly persons (mean age ± SEM: femur 68.6 ± 2.1 years; iliac crest 66 ± 3.1 years). Data were calculated using t test and are presented as mean ± SEM. ^*p < 0.05: PBMC vs. femur derived BMMC; ^§p < 0.05: PBMC vs. iliac crest-derived BMMC. There were no significant differences between samples obtained from the two different sources of BM. ^#P values: femur versus iliac crest.

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