Figure 3From: Increased activation and cytokine secretion in B cells stimulated with leptin in aged humansLeptin-stimulated cytokine secreting B cells. Purified B cells (12,500-50,000/well) from young and aged were cultured in the absence or presence of leptin (25 ng/ml and 50 ng/ml) for 24 hours and TNF-α, IL-6, and IL-10 secreting B cells were analyzed by ELISPOT assay. Data are expressed as mean ± sd. Each experiment was done in triplicates.Back to article page