Table 1 Impact of 30 μM ebselen or 7.5 mM NAC on proliferative capacity and lifespan of TCC supplemented from a young in vitro age
Clone (Age of the donor) | Initial PD | Concentration | Average PD per week | Cumulative PD achieved at the end of lifespan in culture |
|---|---|---|---|---|
400-23 (26 year old donor) | 34.5 | Control | 0.7 | 44.9 |
400-23 (26 year old donor) | 34.5 | 10 μM ebselen | 0.8 | 46.9 |
400-23 (26 year old donor) | 34.5 | 30 μM ebselen | 1.2 | 56.2* |
400-23 (26 year old donor) | 34.5 | 60 μM ebselen | Inhibited growth after one week in culture | 34.5 |
400-23 (26 year old donor) | 34.5 | 80 μM ebselen | Inhibited growth after one week in culture | 34.5 |
400-23 (26 year old donor) | 34.5 | 100 μM ebselen | Inhibited growth after one week in culture | 34.5 |
400-23 (26 year old donor) | 34.5 | Control | 0.9 | 49.1 |
400-23 (26 year old donor) | 34.5 | 1.25 mM NAC | 0.8 | 48.4 |
400-23 (26 year old donor) | 34.5 | 5 mM NAC | 1.1 | 53.5 |
400-23 (26 year old donor) | 34.5 | 7.5 mM NAC | 1.2 | 56.1* |
400-23 (26 year old donor) | 34.5 | 10 mM NAC | Inhibited growth after two weeks in culture | 35.8 |
385-7 (45 year old donor) | 31.0 | Control | 0.9 | 44.4 |
385-7 (45 year old donor) | 31.0 | 10 μM ebselen | 0.9 | 44.9 |
385-7 (45 year old donor) | 31.0 | 30 μM ebselen | 1.4 | 50.8* |
385-7 (45 year old donor) | 31.0 | 60 μM ebselen | Inhibited growth after one week in culture | 31.0 |
385-7 (45 year old donor) | 31.0 | 80 μM ebselen | Inhibited growth after one week in culture | 31.0 |
385-7 (45 year old donor) | 31.0 | 100 μM ebselen | Inhibited growth after one week in culture | 31.0 |
385-7 (45 year old donor) | 31.0 | Control | 1.0 | 47.2 |
385-7 (45 year old donor) | 31.0 | 1.25 mM NAC | 1.0 | 48.7 |
385-7 (45 year old donor) | 31.0 | 5 mM NAC | 1.3 | 52.8 |
385-7 (45 year old donor) | 31.0 | 7.5 mM NAC | 1.4 | 55.8* |
385-7 (45 year old donor) | 31.0 | 10 mM NAC | Inhibited growth after one week in culture | 31.0 |