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Table 1 Impact of 30 μM ebselen or 7.5 mM NAC on proliferative capacity and lifespan of TCC supplemented from a young in vitro age

From: An investigation of the effects of the antioxidants, ebselen or N-acetyl cysteine on human peripheral blood mononuclear cells and T cells

Clone (Age of the donor) Initial PD Concentration Average PD per week Cumulative PD achieved at the end of lifespan in culture
400-23 (26 year old donor) 34.5 Control 0.7 44.9
400-23 (26 year old donor) 34.5 10 μM ebselen 0.8 46.9
400-23 (26 year old donor) 34.5 30 μM ebselen 1.2 56.2*
400-23 (26 year old donor) 34.5 60 μM ebselen Inhibited growth after one week in culture 34.5
400-23 (26 year old donor) 34.5 80 μM ebselen Inhibited growth after one week in culture 34.5
400-23 (26 year old donor) 34.5 100 μM ebselen Inhibited growth after one week in culture 34.5
400-23 (26 year old donor) 34.5 Control 0.9 49.1
400-23 (26 year old donor) 34.5 1.25 mM NAC 0.8 48.4
400-23 (26 year old donor) 34.5 5 mM NAC 1.1 53.5
400-23 (26 year old donor) 34.5 7.5 mM NAC 1.2 56.1*
400-23 (26 year old donor) 34.5 10 mM NAC Inhibited growth after two weeks in culture 35.8
385-7 (45 year old donor) 31.0 Control 0.9 44.4
385-7 (45 year old donor) 31.0 10 μM ebselen 0.9 44.9
385-7 (45 year old donor) 31.0 30 μM ebselen 1.4 50.8*
385-7 (45 year old donor) 31.0 60 μM ebselen Inhibited growth after one week in culture 31.0
385-7 (45 year old donor) 31.0 80 μM ebselen Inhibited growth after one week in culture 31.0
385-7 (45 year old donor) 31.0 100 μM ebselen Inhibited growth after one week in culture 31.0
385-7 (45 year old donor) 31.0 Control 1.0 47.2
385-7 (45 year old donor) 31.0 1.25 mM NAC 1.0 48.7
385-7 (45 year old donor) 31.0 5 mM NAC 1.3 52.8
385-7 (45 year old donor) 31.0 7.5 mM NAC 1.4 55.8*
385-7 (45 year old donor) 31.0 10 mM NAC Inhibited growth after one week in culture 31.0
  1. Values statistically different from their controls (Student’s t-test, 95% confidence level) are indicated with an asterisk. * p < 0.05, Significantly higher than the proliferative capacity of non-supplemented cells.