Figure 2

Insulin signalling in liver of young, old (Old) and exercised old rats (Old Exe). A. The decrease in the glucose disappearance rate (Kitt), induced by the aging associated with obesity, returned to the young rats level 16 h after acute exercise. Liver extracts from rats injected with saline or insulin were prepared as described in the Methods. B, tissue extracts were immunoblotting (IB) with anti-phospho-IRβ antibody (upper panel) or anti-IRβ antibody (lower panel). C, tissue extracts were also IP with anti-IRS-1 antibody and IB with anti-phosphotyrosine (pY) antibody (upper panels), and IB with anti-IRS-1 antibody (lower panel). D, liver extracts were IB with anti-phospho-Akt and anti-Akt antibody (upper and lower panel, respectively). E, liver extracts were IB with anti-phospho-Foxo1 and anti-Foxo1 antibody (upper and lower panel, respectively). The results of scanning densitometry were expressed as arbitrary units. Bars represent means ± S.E.M. of n = 6 rats. ^∗P < 0.05, versus Young rats and ^#p < 0.05 versus Old.