Figure 4

Protein level of PEPCK and G6Pase in the liver of Young, Old and Old Exe groups. Liver extract rats were submitted to immunobloting for PEPCK and G6Pase protein level, as described in Methods. A, tissue extracts were blotted (IB) with anti-PEPCK antibody. B, tissue extracts were blotted (IB) with anti-G6Pase antibody. Immunoblot was performed employing anti-β-Actin antibody as the loaded protein (lower panels in A and B). The results of scanning densitometry are expressed as arbitrary units. Bars represent means ± S.E.M. of n = 6 rats. ^∗P < 0.05, Young rats and ^#P < 0.05, versus Old.