| |
Frequency of cellsa
|
|---|
|
Peripheral blood
|
Day 1
|
Day 5
|
Day 10
| |
|---|
| |
Juvenile
|
Aged
|
Juvenile
|
Aged
|
Juvenile
|
Aged
|
Statistics
|
|---|
|
Monocytes
| | | | | | | |
|
CCR1
|
5.0 ± 0.9
|
1.8 ± 0.4
|
1.0 ± 0.1
|
2.2 ± 1.7
|
2.5 ± 0.3
|
2.4 ± 0.6
|
d.p.i*
|
|
CCR3
|
1.6 ± 0.4
|
1.9 ± 04
|
1.1 ± 01
|
2.2 ± 12
|
1.1 ± 01
|
2.4 ± 0.6
|
age*
|
|
CD86
|
8.9 ± 13
|
3.8 ± 0.4
|
6.3 ± 2.0
|
3.9 ± 1.9
|
4.7 ± 0.3
|
5.2 ± 1.6
|
age*
|
|
DCs
| | | | | | | |
|
CCR6
|
0.6 ± 0.3
|
0.3 ± 0.2
|
0.6 ± 0.2
|
0.0 ± 0.0
|
0.3 ± 01
|
0.2 ± 0.0
|
age**, d.p.i
*
|
|
CD86
|
1.4 ± 0.5
|
0.2 ± 0.0
|
2.9 ± 0.9
|
0.3 ± 0.0
|
0.2 ± 0.0
|
0.2 ± 0.0
|
age****
|
|
CD8 + T cells
| | | | | | | |
|
CCR5
|
6.9 ± 1.2
|
2.5 ± 0.4
|
1.8 ± 0.3
|
2.0 ± 0.5
|
3.7 ± 0.3
|
9.9 ± 3.4
|
d.p.i**
|
|
Lung
|
Mock
|
Day 5
|
Day 10
| |
| |
Juvenile
|
Aged
|
Juvenile
|
Aged
|
Juvenile
|
Aged
|
Statistics
|
|
Macrophages
| | | | | | | |
|
CD86
|
20.5 ± 1.7
|
2.3 ± 0.1
|
36.4 ± 4.8
|
3.1 ± 1.1
|
34.2 ± 2.1
|
3.3 ± 1.7
|
age
****
|
|
DCs
| | | | | | | |
|
CD86
|
1.1 ± 0.3
|
0.3 ± 0.1
|
3.7 ± 0.5
|
0.4 ± 0.1
|
2.0 ± 0.5
|
0.3 ± 0.1
|
age
****
|
- aAverage frequency of chemokine receptor or activation marker positive cells of total perhipheral blood or lung leukocytes. Monocytes (CD14 + HLADR+), peripheral blood dendritic cells (DCs: CD14-HLADR + CD11c+), CD8 T cells (CD3 + CD8+). Lung macrophages are defined as CD68 + HLADR + and lung DCs as CD68-HLADR + CD11c+. Gating strategies are show in Additional file 1: Figure S1, Additional file 2: Figure S2 and Additional file 4: Figure S3. Statistical results of 2-way ANOVas for age and days post infection (d.p.i.) are indicated at the right with asterisks denoting degree of sigrificance: *p < 0.05, **p < 0.005 ****p < 0.0001.
