Figure 6

No T cell lymphopenia in lungs and liver during ageing and no impact of sex on GALT CD4 T cell accumulation. (A) Lungs and liver CD4 and CD8 T cells were recovered from young (Y) (n = 7), middle-aged (M) (n = 7) C57BL/6 mice. Numeration and FACS staining were performed as previously described. (B) Sex dependent analyses of thymocytes (left), pooled secondary lymphoid organs (middle) and colonic lamina propria (right) CD4 T cell numbers. Female animals are represented in dotted columns, male in plain white columns. Cumulative results show the mean ± SEM of absolute numbers recovered from independent experiments comparing young and middle-aged animals. Statistical significance (Student’s t test) is shown: ns, non-significant; *, p < 0.05; **, p < 0.01.