Figure 4
From: Constitutive degradation of IκBα in human T lymphocytes is mediated by calpain
Effect of E64D on stimulus-dependent modification of IκBα (Western Blots). T cells obtained from young and elderly donors were either left untreated or treated with E64D (50 μM) for 45 min. At the end of incubation cells were stimulated with 100 μM pervanadate or TNFα (10 ng/ml) for 10, 20 or 30 min. Cell lysates were prepared and resolved by SDS-PAGE, using equal amounts of protein (30 μg / lane). Resolved proteins were transferred to nitrocellulose membrane and Western blotted with antibody to IκBα and detected using ECL. Representative results obtained from one donor pair are presented. Arrows represent IκBα specific bands detected and used in the analyses.